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Trifolium pratense (Red Clover) | Botanical Profile

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Review & More General Articles on Trifolium pratense (red clover) by the UIC Botanical Center

Piersen CE. Phytoestrogens in botanical dietary supplements: implications for cancer. Integr Cancer Ther. 2003, 2(2):120-38.

Piersen CE, Booth NL, Sun Y, Liang W, Burdette JE, van Breemen RB, Geller SE, Gu C, Banuvar S, Shulman LP, Bolton JL, Farnsworth NR. Chemical and biological characterization and clinical evaluation of botanical dietary supplements: a phase I red clover extract as a model. Curr Med Chem. 2004, 11(11), 1361-74.

van Breemen RB. Development of Safe and Effective Botanical Dietary Supplements. J Med Chem. 2015, 58(21):8360-72.

Dietz BM, Hajirahimkhan A, Dunlap TL, Bolton JL. Botanicals and Their Bioactive Phytochemicals for Women’s Health. Pharmacol Rev. 2016, 68(4), 1026-1073.

Peer-reviewed Publications on Trifolium pratasne (red clover) by the UIC Botanical Center

Total number of publications on this botanical: 19

With direct DOI links to the original articles.

Liu J, Burdette JE, Xu H, Gu C, van Breemen RB, Bhat KP, Booth N, Constantinou AI, Pezzuto JM, Fong HH, Farnsworth NR, Bolton JL. Evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptoms. J Agric Food Chem. 2001, 49(5):2472-9.

Johnson BM, Bolton JL, van Breemen RB. Screening botanical extracts for quinoid metabolites. Chem Res Toxicol. 2001, 14(11), 1546-51.

Xu H, Fabricant DS, Piersen CE, Bolton JL, Pezzuto JM, Fong H, Totura S, Farnsworth NR, Constantinou AI. A preliminary RAPD-PCR analysis of Cimicifuga species and other botanicals used for women’s health. Phytomedicine. 2002, 9(8), 757-62.

Burdette JE, Liu J, Lantvit D, Lim E, Booth N, Bhat KP, Hedayat S, Van Breemen RB, Constantinou AI, Pezzuto JM, Farnsworth NR, Bolton JL. Trifolium pratense (red clover) exhibits estrogenic effects in vivo in ovariectomized Sprague-Dawley rats. J Nutr. 2002, 132(1):27-30.

Overk CR, Yao P, Chadwick LR, Nikolic D, Sun Y, Cuendet MA, Deng Y, Hedayat AS, Pauli GF, Farnsworth NR, van Breemen RB, Bolton JL. Comparison of the in vitro estrogenic activities of compounds from hops (Humulus lupulus) and red clover (Trifolium pratense). J Agric Food Chem. 2005, 53(16), 6246-53.

Booth NL, Overk CR, Yao P, Totura S, Deng Y, Hedayat AS, Bolton JL, Pauli GF, Farnsworth NR. Seasonal variation of red clover (Trifolium pratense L., Fabaceae) isoflavones and estrogenic activity. J Agric Food Chem. 2006, 54(4), 1277-82.

Booth NL, Piersen CE, Banuvar S, Geller SE, Shulman LP, Farnsworth NR. Clinical studies of red clover (Trifolium pratense) dietary supplements in menopause: a literature review. Menopause. 2006, 13(2):251-64.

Booth NL, Overk CR, Yao P, Burdette JE, Nikolic D, Chen SN, Bolton JL, van Breemen RB, Pauli GF, Farnsworth NR. The chemical and biologic profile of a red clover (Trifolium pratense L.) phase II clinical extract. J Altern Complement Med. 2006, 12(2), 133-9.

Overk CR, Guo J, Chadwick LR, Lantvit DD, Minassi A, Appendino G, Chen SN, Lankin DC, Farnsworth NR, Pauli GF, van Breemen RB, Bolton JL. In vivo estrogenic comparisons of Trifolium pratense (red clover), Humulus lupulus (hops), and the pure compounds isoxanthohumol and 8-prenylnaringenin. Chem Biol Interact. 2008, 176(1), 30-9.

Choi Y, van Breemen RB. Development of a screening assay for ligands to the estrogen receptor based on magnetic microparticles and LC-MS. Comb Chem High Throughput Screen. 2008, 11(1), 1-6.

Geller SE, Shulman LP, van Breemen RB, Banuvar S, Zhou Y, Epstein G, Hedayat S, Nikolic D, Krause EC, Piersen CE, Bolton JL, Pauli GF, Farnsworth NR. Safety and efficacy of black cohosh and red clover for the management of vasomotor symptoms: a randomized controlled trial. Menopause. 2009, 16(6), 1156-66.

Phansalkar RS, Simmler C, Bisson J, Chen SN, Lankin DC, McAlpine JB, Niemitz M, Pauli GF. Evolution of Quantitative Measures in NMR: Quantum Mechanical qHNMR Advances Chemical Standardization of a Red Clover (Trifolium pratense) Extract. J Nat Prod. 2017, 80(3):634-647.

Dunlap TL, Howell CE, Mukand N, Chen SN, Pauli GF, Dietz BM, Bolton JL. Red Clover Aryl Hydrocarbon Receptor (AhR) and Estrogen Receptor (ER) Agonists Enhance Genotoxic Estrogen Metabolism. Chem Res Toxicol. 2017, 30(11):2084-2092.

Malca Garcia GR, Friesen JB, Liu Y, Nikolić D, Lankin DC, McAlpine JB, Chen SN, Pauli GF. Preparation of DESIGNER extracts of red clover (Trifolium pratense L.) by centrifugal partition chromatography. J Chromatogr A. 2019, 1605, 360277.

Malca-Garcia GR, Zagal D, Graham J, Nikolić D, Friesen JB, Lankin DC, Chen SN, Pauli GF. Dynamics of the isoflavone metabolome of traditional preparations of Trifolium pratense L. J Ethnopharmacol. 2019, 238:111865.

Muchiri RN, van Breemen RB. Single-Laboratory Validation of UHPLC-MS/MS Assays for Red Clover Isoflavones in Human Serum and Dietary Supplements. J AOAC Int. 2020, 103(4):1160-1166.

Chen L, Choi J, Leonard SW, Banuvar S, Barengolts E, Viana M, Chen SN, Pauli GF, Bolton JL, van Breemen RB. No Clinically Relevant Pharmacokinetic Interactions of a Red Clover Dietary Supplement with Cytochrome P450 Enzymes in Women. J Agric Food Chem. 2020, 68(47), 13929-13939.

Malca-Garcia GR, Liu Y, Dong H, Nikolić D, Friesen JB, Lankin DC, McAlpine J, Chen SN, Dietz BM, Pauli GF. Auto-hydrolysis of red clover as “green” approach to (iso)flavonoid enriched products. Fitoterapia. 2021, 152:104878.

Malca-Garcia GR, Liu Y, Nikolić D, Friesen JB, Lankin DC, McAlpine JB, Chen SN, Pauli GF. Investigation of red clover (Trifolium pratense) Isoflavonoid residual complexity by off-line CCS-qHNMR. Fitoterapia. 2021, 105016.